KMID : 1007520100190051347
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Food Science and Biotechnology 2010 Volume.19 No. 5 p.1347 ~ p.1353
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Protective Effects of Extract with Phenolics from Camellia (Camellia japonica) Leaf against Oxidative Stress-induced Neurotoxicity
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Jeong Chang-Ho
Kim Ji-Hye Choi Gwi-Nam Kwak Ji-Hyun Kim Dae-Ok Heo Ho-Jin
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Abstract
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Antioxidant and neuronal cell protective effects of aqueous extract from camellia (Camellia japonica) leaf (CJLE) were evaluated. The 1,1-phenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing/antioxidant power (FRAP) assays of the CJLE were increased in a dose dependent manner. In neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), the aqueous extract showed protective effect against H2O2-induced neurotoxicity and lactate dehydrogenase (LDH) release into medium was also inhibited by CJLE (7.13-43.89%). The cell viability of CJLE was higher than vitamin C (200 ¥ìM) by neutral red uptake (NRU) assay at a concentration of 250-1,000 ¥ìg/ mL. Phenolics of CJLE were 21.75mg/g, and major phenolic compounds were quercetin (120.20mg/100 g) and kaempferol (88.13 mg/100 g). Therefore these data suggested that the CJLE including above phenolics may be useful in the natural antioxidant substance and reduce the risk of neurodegenerative disease such as Alzheimer¡¯s disease.
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KEYWORD
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camellia leaf, antioxidant activity, neuronal cell protective effect, quercetin, kaempferol
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